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Recombinant Human NR5A1 293 Cell Lysate

Cat.No.: NR5A1-3705HCL
Description: Antigen standard for nuclear receptor subfamily 5, group A, member 1 (NR5A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NR5A1 nuclear receptor subfamily 5, group A, member 1 [ Homo sapiens ]
Official Symbol: NR5A1
Synonyms: NR5A1; nuclear receptor subfamily 5, group A, member 1; FTZF1; steroidogenic factor 1; AD4BP; ELP; FTZ1; SF 1; STF-1; nuclear receptor AdBP4; steroidogenic factor-1; adrenal 4 binding protein; adrenal 4-binding protein; fushi tarazu factor homolog 1; steroid hormone receptor Ad4BP; steroidogenic factor 1 nuclear receptor; SF1; POF7; SF-1; SPGF8; SRXY3;
Gene ID: 2516
mRNA Refseq: NM_004959
Protein Refseq: NP_004950
UniProt ID: Q13285
Chromosome Location: 9q33
Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem; Nuclear Receptors, organism-specific biosystem; Ovarian Infertility Genes, organism-specific biosystem; Wnt Signaling Pathway NetPath, organism-specific biosystem;
Function: DNA binding; DNA binding; chromatin binding; double-stranded DNA binding; enzyme binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; phospholipid binding; protein binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; steroid hormone receptor activity; transcription coactivator activity; zinc ion binding;

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