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Recombinant Human NEUROG1 293 Cell Lysate

Cat.No.: NEUROG1-3865HCL
Description: Antigen standard for neurogenin 1 (NEUROG1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NEUROG1 neurogenin 1 [ Homo sapiens ]
Official Symbol: NEUROG1
Synonyms: NEUROG1; neurogenin 1; NEUROD3; neurogenin-1; AKA; bHLHa6; Math4C; neurogenic differentiation 3; ngn1; NGN-1; neurogenic differentiation factor 3; class A basic helix-loop-helix protein 6; neurogenic basic-helix-loop-helix protein;
Gene ID: 4762
mRNA Refseq: NM_006161
Protein Refseq: NP_006152
MIM: 601726
UniProt ID: Q92886
Chromosome Location: 5q23-q31
Pathway: Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem;
Function: DNA binding; E-box binding; chromatin binding; sequence-specific DNA binding transcription factor activity; transcription factor binding transcription factor activity;

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