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Recombinant Human NANOG 293 Cell Lysate

Cat.No. : NANOG-3981HCL
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  • Gene Information
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Description : Antigen standard for Nanog homeobox (NANOG) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : NANOG Nanog homeobox [ Homo sapiens ]
Official Symbol : NANOG
Synonyms : NANOG; Nanog homeobox; homeobox protein NANOG; FLJ12581; FLJ40451; hNanog; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48;
Gene ID : 79923
mRNA Refseq : NM_024865
Protein Refseq : NP_079141
MIM : 607937
UniProt ID : Q9H9S0
Chromosome Location : 12p13.31
Pathway : Wnt Signaling Pathway and Pluripotency, organism-specific biosystem;
Function : DNA binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Are there any disease associations linked to NANOG dysregulation? 08/04/2021

Dysregulation of NANOG expression has been implicated in various diseases, including cancer and developmental disorders. Aberrant NANOG expression can promote tumor initiation, progression, and metastasis, as well as contribute to embryonic developmental abnormalities.

How does NANOG contribute to immune regulation and tolerance? 07/25/2020

NANOG has been shown to play a role in immune regulation and tolerance by promoting the generation of regulatory T cells (Tregs) and inhibiting the differentiation of effector T cells. It modulates the expression of genes involved in Treg development and function, thereby contributing to immune homeostasis.

What are the regulatory mechanisms controlling NANOG expression? 07/19/2020

NANOG expression is regulated by a complex network of transcription factors, epigenetic modifications, and signaling pathways. Transcription factors like OCT4, SOX2, and KLF4 bind to the NANOG promoter region, while epigenetic modifications such as DNA methylation and histone acetylation also influence its expression.

What is the cellular localization of NANOG protein in the body? 02/21/2020

NANOG protein is predominantly localized in the nucleus of embryonic stem cells (ESCs) and in the inner cell mass of preimplantation embryos. Its nuclear localization is essential for its role in maintaining pluripotency and self-renewal in ESCs.

What potential therapeutic implications does NANOG hold? 02/13/2018

Understanding the role of NANOG in pluripotency and disease pathogenesis opens up potential therapeutic avenues. Targeting NANOG and its associated signaling pathways may provide strategies for regenerative medicine, cancer therapy, and the treatment of developmental disorders. Further research is needed to fully explore the therapeutic potential of NANOG modulation.

How is the expression of NANOG regulated during embryonic development and cellular differentiation? 12/01/2017

The expression of NANOG is tightly controlled during embryonic development and cellular differentiation. Various signaling pathways, including the Wnt, BMP, and FGF pathways, are involved in regulating NANOG expression. Additionally, epigenetic modifications, such as DNA methylation and histone modifications, can also influence NANOG expression. During cellular differentiation, NANOG expression is generally downregulated as cells acquire specialized identities. Transcription factors and microRNAs also play important roles in regulating NANOG expression and mediating its temporal and spatial expression patterns.

What are the signaling pathways regulated by NANOG? 02/14/2017

NANOG regulates several signaling pathways involved in pluripotency, including the Wnt/β-catenin, TGF-β/Smad, and PI3K/AKT pathways. It interacts with key components of these pathways, modulating their activity to maintain the pluripotent state of stem cells.

Customer Reviews (3)

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Reviews
03/31/2022

    High efficiency characterizes the use of this experimental reagent, rendering my research work seamless and productive.

    10/03/2018

      The operation of the reagent is straightforward, without the need for additional equipment or materials.

      04/13/2018

        High quality and stability ensure the accuracy and reliability of my experimental results while using this reagent.

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