"MAFF" Related Products

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Recombinant Human MAFF 293 Cell Lysate

Cat.No.: MAFF-4560HCL
Description: Antigen standard for v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian) (MAFF), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MAFF v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian) [ Homo sapiens ]
Official Symbol: MAFF
Synonyms: MAFF; v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian); v maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein F; transcription factor MafF; hMafF; v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F; U-MAF;
Gene ID: 23764
mRNA Refseq: NM_001161572
Protein Refseq: NP_001155044
MIM: 604877
UniProt ID: Q9ULX9
Chromosome Location: 22q13.1
Pathway: Factors involved in megakaryocyte development and platelet production, organism-specific biosystem; Hemostasis, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem;
Function: sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity;

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