Recombinant Human LATS2 293 Cell Lysate

• Cat.No.: LATS2-4813HCL

Specification

• Description: Antigen standard for LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: LATS2 LATS, large tumor suppressor, homolog 2 (Drosophila) [ Homo sapiens ]
• Official Symbol: LATS2
• Synonyms: LATS2; LATS, large tumor suppressor, homolog 2 (Drosophila); LATS (large tumor suppressor, Drosophila) homolog 2; serine/threonine-protein kinase LATS2; warts-like kinase; serine/threonine kinase KPM; large tumor suppressor homolog 2; serine/threonine-protein kinase kpm; kinase phosphorylated during mitosis protein; KPM; FLJ13161
• Gene ID: 26524
• mRNA Refseq: NM_014572
• Protein Refseq: NP_055387
• MIM: 604861
• UniProt ID: Q9NRM7
• Chromosome Location: 13q11-q12
• Pathway: Coregulation of Androgen receptor activity, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by Hippo, organism-specific biosystem
• Function: ATP binding; metal ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity