Recombinant Human Protein Kinase C, Beta, 2-673aa, His-tagged

• Cat.No.: PRKCB-60H
• Product Overview: Recombinant Human protein kinase C, beta produced inSf9is a glycosylated, polypeptide chain containing amino acids 2-673 and having a molecular mass of 80 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag. PKC-is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: PRKCB-60H
• Description: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
• Source: Sf9 insect cells.
• Unit Definition: The Unit Definition is 780units/mg. One unit of PKCβΙΙ activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30°C. Recombinant human active PKCβΙΙ also phosphorylates full length myelin basic protein, histone, HIV Tat-1, GSK-3β and cdk7. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Formulation: PKC-is supplied at a of 0.1mg/ml in 10mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: PRKCB protein kinase C, beta [ Homo sapiens ]
• Synonyms: PRKCB; protein kinase C, beta;PKCB; PRKCB1; PRKCB2; MGC41878; PKC-beta;Protein kinase C beta type;EC2.7.11.13; PKC-B; protein kinase C, beta 1; protein kinase C, beta 1 polypeptide
• Gene ID: 5579
• mRNA Refseq: NM_002738
• Protein Refseq: NP_002729
• UniProt ID: P05771
• Chromosome Location: 16p12
• MIM: 176970
• Pathway: B cell receptor signaling pathway; Calcium signaling pathway; Chemokine signaling pathway; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; GnRH signaling pathway; Leukocyte transendothelial migration; Long-term depression; Long-term potentiation; Non-small cell lung cancer; Pathways in cancer; Phosphatidylinositol signaling system; Tight junction; VEGF signaling pathway; Vascular smooth muscle contraction; Vibrio cholerae infection; Wnt signaling pathway; Hemostasis; Synaptic Transmission
• Function: ATP binding; calcium ion binding; nucleotide binding; protein binding; protein kinase C activity; transferase activity; zinc ion binding