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Recombinant Human ITGB1BP1 293 Cell Lysate

Cat.No.: ITGB1BP1-5126HCL
Description: Antigen standard for integrin beta 1 binding protein 1 (ITGB1BP1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ITGB1BP1 integrin beta 1 binding protein 1 [ Homo sapiens ]
Official Symbol: ITGB1BP1
Synonyms: ITGB1BP1; integrin beta 1 binding protein 1; integrin beta-1-binding protein 1; bodenin; ICAP 1A; ICAP 1alpha; ICAP 1B; ICAP1; ICAP1A; ICAP1B; integrin cytoplasmic domain associated protein 1; integrin cytoplasmic domain associated protein 1 alpha; integrin cytoplasmic domain associated protein 1 beta; ICAP-1; integrin cytoplasmic domain-associated protein 1; integrin cytoplasmic domain-associated protein 1-beta; integrin cytoplasmic domain-associated protein 1-alpha; ICAP-1A; ICAP-1B; ICAP-1alpha; DKFZp686K08158;
Gene ID: 9270
mRNA Refseq: NM_004763
Protein Refseq: NP_004754
MIM: 607153
UniProt ID: O14713
Chromosome Location: 2p25.2
Function: protein binding;

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