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Recombinant Human INPP5K 293 Cell Lysate

Cat.No. : INPP5K-5196HCL
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  • Gene Information
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Description : Antigen standard for inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : INPP5K inositol polyphosphate-5-phosphatase K [ Homo sapiens ]
Official Symbol : INPP5K
Synonyms : INPP5K; inositol polyphosphate-5-phosphatase K; inositol polyphosphate 5-phosphatase K; skeletal muscle and kidney enriched inositol phosphatase; SKIP; skeletal muscle and kidney-enriched inositol phosphatase; PPS;
Gene ID : 51763
mRNA Refseq : NM_016532
Protein Refseq : NP_057616
MIM : 607875
UniProt ID : Q9BT40
Chromosome Location : 17p13.3
Pathway : 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian), organism-specific biosystem; 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian), conserved biosystem; 3-phosphoinositide degradation, organism-specific biosystem; 3-phosphoinositide degradation, conserved biosystem; D-myo-inositol (1,3,4)-trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (1,3,4)-trisphosphate biosynthesis, conserved biosystem; D-myo-inositol (1,4,5)-trisphosphate degradation, organism-specific biosystem;
Function : hydrolase activity; inositol bisphosphate phosphatase activity; inositol trisphosphate phosphatase activity; inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity; inositol-1,4,5-trisphosphate 5-phosphatase activity; inositol-polyphosphate 5-phosphatase activity; lipid phosphatase activity; phosphatidylinositol phosphate 5-phosphatase activity; phosphatidylinositol trisphosphate phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity; protein binding; vasopressin receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the clinical significance of d-3-PHGDH alterations? 10/09/2022

Mutations in d-3-PHGDH have been associated with a neurological phenotype that overlaps with Marinesco-Sjögren syndrome and INPP5K disease.

What potential therapeutic strategy is mentioned for patients with d-3-PHGDH mutations? 09/12/2022

l-serine administration is proposed as a promising therapeutic strategy for patients with d-3-PHGDH mutations.

What does this study reveal about the three rare diseases under investigation? 03/22/2022

This study reveals a common core phenotypical feature and a shared molecular mechanism among Marinesco-Sjögren syndrome, INPP5K disease, and d-3-PHGDH mutations.

What common molecular feature was identified in both Marinesco-Sjögren syndrome and INPP5K patients? 01/20/2022

Alterations in d-3-PHGDH, an enzyme that modulates the production of l-serine, were identified as a common molecular feature.

How many new INPP5K patients were included in this study? 08/10/2020

Six new INPP5K patients were included in this study.

How was the effect of l-serine tested in this study? 05/11/2018

The effect of l-serine was tested in zebrafish models with mutations in sil1, phgdh, and inpp5k a+b genes, which improved their neuronal phenotype.

What disorders are associated with INPP5K mutations? 02/08/2018

INPP5K mutations are associated with a rare disorder that shares symptoms with Marinesco-Sjögren syndrome, such as cataracts, myopathy, and ataxia.

Customer Reviews (3)

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Reviews
04/06/2022

    We observed minimal changes in results even after prolonged storage, showcasing its long-term stability.

    10/28/2019

      Its reliability over time reduced the need for frequent reordering, saving both time and resources.

      04/01/2017

        The absence of adverse effects on cells or organisms underscored the product's safety and suitability for biological research.

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