Recombinant Human INPP5K 293 Cell Lysate
Cat.No. : | INPP5K-5196HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | INPP5K inositol polyphosphate-5-phosphatase K [ Homo sapiens ] |
Official Symbol : | INPP5K |
Synonyms : | INPP5K; inositol polyphosphate-5-phosphatase K; inositol polyphosphate 5-phosphatase K; skeletal muscle and kidney enriched inositol phosphatase; SKIP; skeletal muscle and kidney-enriched inositol phosphatase; PPS; |
Gene ID : | 51763 |
mRNA Refseq : | NM_016532 |
Protein Refseq : | NP_057616 |
MIM : | 607875 |
UniProt ID : | Q9BT40 |
Chromosome Location : | 17p13.3 |
Pathway : | 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian), organism-specific biosystem; 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian), conserved biosystem; 3-phosphoinositide degradation, organism-specific biosystem; 3-phosphoinositide degradation, conserved biosystem; D-myo-inositol (1,3,4)-trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (1,3,4)-trisphosphate biosynthesis, conserved biosystem; D-myo-inositol (1,4,5)-trisphosphate degradation, organism-specific biosystem; |
Function : | hydrolase activity; inositol bisphosphate phosphatase activity; inositol trisphosphate phosphatase activity; inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity; inositol-1,4,5-trisphosphate 5-phosphatase activity; inositol-polyphosphate 5-phosphatase activity; lipid phosphatase activity; phosphatidylinositol phosphate 5-phosphatase activity; phosphatidylinositol trisphosphate phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity; protein binding; vasopressin receptor activity; |
Products Types
◆ Recombinant Protein | ||
INPP5K-2098R | Recombinant Rhesus Macaque INPP5K Protein, His (Fc)-Avi-tagged | +Inquiry |
Inpp5k-3550M | Recombinant Mouse Inpp5k Protein, Myc/DDK-tagged | +Inquiry |
INPP5K-5104H | Recombinant Human INPP5K Protein, GST-tagged | +Inquiry |
INPP5K-5448C | Recombinant Chicken INPP5K | +Inquiry |
INPP5K-2277R | Recombinant Rhesus monkey INPP5K Protein, His-tagged | +Inquiry |
◆ Lysates | ||
INPP5K-5197HCL | Recombinant Human INPP5K 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (7)
Ask a questionMutations in d-3-PHGDH have been associated with a neurological phenotype that overlaps with Marinesco-Sjögren syndrome and INPP5K disease.
l-serine administration is proposed as a promising therapeutic strategy for patients with d-3-PHGDH mutations.
This study reveals a common core phenotypical feature and a shared molecular mechanism among Marinesco-Sjögren syndrome, INPP5K disease, and d-3-PHGDH mutations.
Alterations in d-3-PHGDH, an enzyme that modulates the production of l-serine, were identified as a common molecular feature.
Six new INPP5K patients were included in this study.
The effect of l-serine was tested in zebrafish models with mutations in sil1, phgdh, and inpp5k a+b genes, which improved their neuronal phenotype.
INPP5K mutations are associated with a rare disorder that shares symptoms with Marinesco-Sjögren syndrome, such as cataracts, myopathy, and ataxia.
Customer Reviews (3)
Write a reviewWe observed minimal changes in results even after prolonged storage, showcasing its long-term stability.
Its reliability over time reduced the need for frequent reordering, saving both time and resources.
The absence of adverse effects on cells or organisms underscored the product's safety and suitability for biological research.
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