"ICT1" Related Products

Recombinant Human ICT1 293 Cell Lysate

Cat.No.: ICT1-5313HCL
Description: Antigen standard for immature colon carcinoma transcript 1 (ICT1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ICT1 immature colon carcinoma transcript 1 [ Homo sapiens ]
Official Symbol: ICT1
Synonyms: ICT1; immature colon carcinoma transcript 1; peptidyl-tRNA hydrolase ICT1, mitochondrial; DS 1; digestion substraction 1; immature colon carcinoma transcript 1 protein; DS1; DS-1;
Gene ID: 3396
mRNA Refseq: NM_001545
Protein Refseq: NP_001536
MIM: 603000
UniProt ID: Q14197
Chromosome Location: 17q25
Function: aminoacyl-tRNA hydrolase activity; hydrolase activity; translation release factor activity, codon nonspecific;

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