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Recombinant Human GOT1 293 Cell Lysate

Cat.No.: GOT1-5824HCL
Description: Antigen standard for glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GOT1 glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) [ Homo sapiens ]
Official Symbol: GOT1
Synonyms: GOT1; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); aspartate aminotransferase, cytoplasmic; transaminase A; growth-inhibiting protein 18; glutamate oxaloacetate transaminase 1; GIG18; ASTQTL1;
Gene ID: 2805
mRNA Refseq: NM_002079
Protein Refseq: NP_002070
MIM: 138180
UniProt ID: P17174
Chromosome Location: 10q24.1-q25.1
Pathway: Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Cysteine and methionine metabolism, organism-specific biosystem;
Function: L-aspartate:2-oxoglutarate aminotransferase activity; L-aspartate:2-oxoglutarate aminotransferase activity; L-aspartate:2-oxoglutarate aminotransferase activity; L-phenylalanine:2-oxoglutarate aminotransferase activity; carboxylic acid binding; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding; transaminase activity;

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