Recombinant Human TEK Tyrosine Kinase, Endothelial, Fc-tagged
Cat.No. : | TEK-75H |
Product Overview : | Recombinant Human Soluble TIE-2 fused with the Fc part of human IgG1 produced in baculovirus is a monomeric, glycosylated, polypeptide containing 730 amino acids and having a total molecular mass of 250 kDa. Human TIE-2/Fc monomer has a calculated molecular mass of approximately 125 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 140 kDa protein in SDS-PAGE under reducing conditions. The TIE2 Fc Chimera is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | TEK-75H |
Description : | TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1122 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 726 residue extracellular domain and a 353 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-1 display similar angiogenic defects. |
Source : | Insect Cells |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | TIE-2 Fc Chimera was lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS. |
Solubility : | It is recommended to reconstitute the lyophilized TIE-2 Fc Chimera in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Biological Activity : | Measured in a functional ELISA assay. When TIE-2/Fc is immobilized at 4 µg/mL (100 µl/well), it binds rh Angiopoietin-2 with a linear range of 2 -100 ng/ml. |
Storage : | Lyophilized sTIE-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIE-2 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Gene Name : | TEK TEK tyrosine kinase, endothelial [ Homo sapiens ] |
Synonyms : | TEK; TEK tyrosine kinase, endothelial; TIE2; VMCM; TIE-2; VMCM1; CD202B; soluble TIE2 variant 1; soluble TIE2 variant 2; EC 2.7.10.1; venous malformations, multiple cutaneous and mucosal; CD202b; hTIE2; Tyrosine-protein kinase receptor TIE-2; Tyrosine-protein kinase receptor TEK; Tunica interna endothelial cell kinase; p140 TEK; VMCM1; CD202b antigen |
Gene ID : | 7010 |
mRNA Refseq : | NM_000459 |
Protein Refseq : | NP_000450 |
MIM : | 600221 |
UniProt ID : | Q02763 |
Chromosome Location : | 9p21 |
Pathway : | Hemostasis; Signaling in Immune system |
Function : | ATP binding; nucleotide binding; protein binding; receptor activity; transferase activity; transmembrane receptor protein tyrosine kinase activity |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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