"EIF2C3" Related Products

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Recombinant Human EIF2C3 293 Cell Lysate

Cat.No.: EIF2C3-6666HCL
Description: Antigen standard for eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: EIF2C3 eukaryotic translation initiation factor 2C, 3 [ Homo sapiens ]
Official Symbol: EIF2C3
Synonyms: EIF2C3; eukaryotic translation initiation factor 2C, 3; protein argonaute-3; AGO3; argonaute 3; FLJ12765; hAgo3; eIF2C 3; eIF-2C 3; argonaute3; MGC86946;
Gene ID: 192669
mRNA Refseq: NM_024852
Protein Refseq: NP_079128
MIM: 607355
UniProt ID: Q9H9G7
Chromosome Location: 1p34
Pathway: Gene Expression, organism-specific biosystem; MicroRNA (miRNA) Biogenesis, organism-specific biosystem; Post-transcriptional Silencing By Small RNAs, organism-specific biosystem; Pre-NOTCH Expression and Processing, organism-specific biosystem; Pre-NOTCH Transcription and Translation, organism-specific biosystem; Regulatory RNA pathways, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
Function: RNA binding; protein binding; translation initiation factor activity;

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