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Recombinant Human DUS4L 293 Cell Lysate

Cat.No.: DUS4L-6787HCL
Description: Antigen standard for dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DUS4L dihydrouridine synthase 4-like (S. cerevisiae) [ Homo sapiens ]
Official Symbol: DUS4L
Synonyms: DUS4L; dihydrouridine synthase 4-like (S. cerevisiae); tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like; DUS4; PP35; protein similar to E.coli yhdg and R. capsulatus nifR3; 2310069P03Rik; MGC133233;
Gene ID: 11062
mRNA Refseq: NM_181581
Protein Refseq: NP_853559
UniProt ID: O95620
Chromosome Location: 7q22-q31
Function: flavin adenine dinucleotide binding; oxidoreductase activity; tRNA dihydrouridine synthase activity;

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