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Recombinant Human DNAJB1 293 Cell Lysate

Cat.No.: DNAJB1-6892HCL
Description: Antigen standard for DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DNAJB1 DnaJ (Hsp40) homolog, subfamily B, member 1 [ Homo sapiens ]
Official Symbol: DNAJB1
Synonyms: DNAJB1; DnaJ (Hsp40) homolog, subfamily B, member 1; HSPF1; dnaJ homolog subfamily B member 1; Hsp40; radial spoke 16 homolog B (Chlamydomonas); RSPH16B; Sis1; hDj-1; human DnaJ protein 1; heat shock protein 40; dnaJ protein homolog 1; heat shock 40kD protein 1; radial spoke 16 homolog B; heat shock 40 kDa protein 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; Hdj1;
Gene ID: 3337
mRNA Refseq: NM_006145
Protein Refseq: NP_006136
MIM: 604572
UniProt ID: P25685
Chromosome Location: 19p13.2
Pathway: Influenza A, organism-specific biosystem; Influenza A, conserved biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem;
Function: heat shock protein binding; unfolded protein binding;

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