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Recombinant Human DCLRE1C 293 Cell Lysate

Cat.No.: DCLRE1C-7049HCL
Description: Antigen standard for DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae) (DCLRE1C), transcript variant b is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DCLRE1C DNA cross-link repair 1C [ Homo sapiens ]
Official Symbol: DCLRE1C
Synonyms: DCLRE1C; DNA cross-link repair 1C; DNA cross link repair 1C (PSO2 homolog, S. cerevisiae) , SCIDA, severe combined immunodeficiency, type a (Athabascan); protein artemis; A SCID; ARTEMIS; FLJ11360; PSO2 homolog (S. cerevisiae); SNM1C; PSO2 homolog; SNM1 homolog C; SNM1-like protein; DNA cross-link repair 1C protein; severe combined immunodeficiency, type a (Athabascan); DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae); SCIDA; A-SCID; hSNM1C; RS-SCID; DCLREC1C; FLJ36438;
Gene ID: 64421
mRNA Refseq: NM_001033855
Protein Refseq: NP_001029027
MIM: 605988
UniProt ID: Q96SD1
Chromosome Location: 10p13
Pathway: Non-homologous end-joining, organism-specific biosystem; Non-homologous end-joining, conserved biosystem; Primary immunodeficiency, organism-specific biosystem; Primary immunodeficiency, conserved biosystem;
Function: 5-3 exonuclease activity; endonuclease activity; hydrolase activity; single-stranded DNA specific endodeoxyribonuclease activity;

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