"NTPCR" Related Products

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Recombinant Human C1orf57 293 Cell Lysate

Cat.No.: NTPCR-8153HCL
Description: Antigen standard for chromosome 1 open reading frame 57 (C1orf57) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NTPCR nucleoside-triphosphatase, cancer-related [ Homo sapiens ]
Official Symbol: NTPCR
Synonyms: C1orf57; HCR-NTPase; cancer-related nucleoside-triphosphatase; NTPase; human cancer-related NTPase; nucleoside-triphosphatase C1orf57; nucleoside triphosphate phosphohydrolase
Gene ID: 84284
mRNA Refseq: NM_032324
Protein Refseq: NP_115700
UniProt ID: Q9BSD7
Chromosome Location: 1q42.2
Pathway: Purine metabolism, organism-specific biosystem; Thiamine metabolism, organism-specific biosystem; pyrimidine deoxyribonucleotides biosynthesis from CTP, conserved biosystem
Function: ATP binding; nucleoside-triphosphatase activity; nucleotide phosphatase activity, acting on free nucleotides

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