"ARFIP2" Related Products

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Recombinant Human ARFIP2 293 Cell Lysate

Cat.No.: ARFIP2-8749HCL
Description: Antigen standard for ADP-ribosylation factor interacting protein 2 (ARFIP2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ARFIP2 ADP-ribosylation factor interacting protein 2 [ Homo sapiens ]
Official Symbol: ARFIP2
Synonyms: ARFIP2; ADP-ribosylation factor interacting protein 2; arfaptin-2; arfaptin 2; POR1; partner of RAC1 (arfaptin 2); FLJ18046; FLJ18697; FLJ99239;
Gene ID: 23647
mRNA Refseq: NM_001242854
Protein Refseq: NP_001229783
MIM: 601638
UniProt ID: P53365
Chromosome Location: 11p15
Pathway: Arf1 pathway, organism-specific biosystem;
Function: GTP binding; GTP-dependent protein binding; GTP-dependent protein binding; Rac GTPase binding; protein binding; protein domain specific binding;

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