Active Recombinant Human PCSK1, His-tagged

• Cat.No.: PCSK1-180H
• Product Overview: Recombinant Human PCSK1 /NEC1 /Prohormone Convertase 1 Protein (rh PCSK1 /NEC1) Lys 28 - Arg 617 (Accession # AAI30296) was produced in human 293 cells (HEK293).

Specification

• Description: Prohormone convertase 1 (PCSK1) is also known as Neuroendocrine convertase 1 (NEC1), Proprotein convertase 1 (PC1), which belongs to the peptidase S8 family and Furin subfamily. PCSK1 involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK1 / NEC1 release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds. The catalytic substrates of PCSK1 include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, acetyl-Arg-Glu-Lys-Arg-7-amino-trifluoromethyl coumarin (Ac-REKR-AFC).
• Molecular Mass: rh PCSK1 /NEC1 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 57 kDa (Mature form). The predicted N-terminus is Ser 111. DTT-reduced Protein migrates as 66 kDa in SDS-PAGE due to glycosylation and autocatalytic processing.
• Endotoxin: Less than 1.0 EU per μg of the rh PCSK1 /NEC1 by the LAL method.
• Purity: >85% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.

Gene Information

• Gene Name: PCSK1 proprotein convertase subtilisin/kexin type 1 [ Homo sapiens ]
• Official Symbol: PCSK1
• Synonyms: PCSK1; proprotein convertase subtilisin/kexin type 1; NEC1; neuroendocrine convertase 1; PC1; PC3; prohormone convertase 1; prohormone convertase 3; proprotein convertase 1; SPC3; NEC 1; BMIQ12
• Gene ID: 5122
• mRNA Refseq: NM_000439
• Protein Refseq: NP_000430
• MIM: 162150
• UniProt ID: P29120
• Chromosome Location: 5q15-q21
• Pathway: Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Insulin Synthesis and Processing, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem
• Function: chaperone binding; endopeptidase activity; insulin binding; peptidase activity; serine-type endopeptidase activity; serine-type endopeptidase activity