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Active Recombinant Human CD40 protein, His/Fc-tagged, Biotinylated

Cat.No. : CD40-202H
Product Overview : Recombinant Human CD40(Leu 25 - Ser 645) fused with His and human IgG1 Fc fragment tag at C-terminal was expressed in HEK293, was enzymatically single-point biotinlabeled at C-terminus to retain its native conformational structure and bioactivity.
  • Specification
  • Gene Information
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Source : HEK293
Species : Human
Tag : His/Fc
Predicted N Terminal : Glu 21
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Human CD40 Ligand, His Tag at 5 μg/mL ( 100 μl/well ) can bind Biotinylated Human CD40, His Tag & Fc Tag with a linear range of 0.2-1μg/mL.
Molecular Mass : Has a calculated MW of 48 kDa. DTT-reduced Protein migrates as 55-65 kDa in SDS-PAGE due to glycosylation.
Endotoxin : Less than 1.0 EU per μg of the Biotinylated Human CD40, His Tag & Fc tag by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Applications : Has been used successfully for ELISA, western blotting, inhibition assay, pull-down, SPR, FACS, and affinity purification, together with anti-biotin antibody, streptavidin or avidin-conjugated probes.
Storage : Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
Gene Name : CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ]
Official Symbol : CD40
Synonyms : CD40; CD40 molecule, TNF receptor superfamily member 5; TNFRSF5, tumor necrosis factor receptor superfamily, member 5; tumor necrosis factor receptor superfamily member 5; Bp50; p50; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily, member 5; nerve growth factor receptor-related B-lymphocyte activation molecule; CDW40; TNFRSF5; MGC9013;
Gene ID : 958
mRNA Refseq : NM_001250
Protein Refseq : NP_001241
MIM : 109535
UniProt ID : P25942
Chromosome Location : 20q12-q13.2
Pathway : Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem;
Function : enzyme binding; protein binding; receptor activity; signal transducer activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can CD40-targeted therapies be used in the treatment of infectious diseases? 05/31/2022

CD40 agonists have the potential to enhance immune responses against infectious agents, but their clinical use in this context is an area of ongoing research.

Are there any approved drugs targeting CD40 for clinical use? 05/07/2021

As of my knowledge cutoff date in January 2022, there were no FDA-approved drugs specifically targeting CD40. However, clinical trials were ongoing for CD40-targeted therapies.

Can CD40-targeted therapies be used in combination with other cancer treatments? 04/24/2019

Yes, CD40-targeted therapies are often investigated in combination with other treatments like checkpoint inhibitors or chemotherapy to enhance their effectiveness.

What are the potential side effects of CD40-targeted therapies in cancer treatment? 09/25/2018

Side effects may include flu-like symptoms, cytokine release syndrome, and immune-related adverse events, which can be managed with supportive care and close monitoring.

Are there ongoing clinical trials exploring CD40-targeted therapies for atherosclerosis? 04/25/2017

Research on CD40-targeted therapies for atherosclerosis is still in the experimental stages and not yet in advanced clinical trials.

Customer Reviews (3)

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Reviews
10/28/2021

    Its utilization in this technique has allowed for precise visualization and determination of protein structures with remarkable clarity and resolution.

    08/11/2021

      The reliable and high-quality results obtained using CD40 protein make it an excellent choice for researchers studying protein structures.

      10/07/2018

        CD40 protein has proved to be an invaluable tool in protein electron microscopy structure analysis.

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