Active Recombinant Human NUAK2, GST-tagged

• Cat.No.: NUAK2-1068H
• Product Overview: Recombinant full-length human NUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2. NUAK2 is activated by muscle contraction and is a unique mediator of contraction-stimulated glucose transport in skeletal muscle. NUAK2 is involved in cellular stress responses linked to obesity and type 2 diabetes. NUAK2 shows kinase activity against a synthetic test peptide and the activity in keratinocytes is increased by AMP and 5-amino-4-imidazolecarboxamide riboside, implying that AMPK kinase-dependent pathway can activate NAUK2. Glucose deprivation also increases NAUK2 activity in baby hamster kidney fibroblasts.
• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 80 nmol/min/mg
• Molecular Mass: ~110 kDa
• Protein length: Full Length
• Purity: >75%
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: NUAK2 NUAK family, SNF1-like kinase, 2 [ Homo sapiens (human) ]
• Official Symbol: NUAK2
• Synonyms: NUAK2; NUAK family, SNF1-like kinase, 2; NUAK family SNF1-like kinase 2; FLJ90349; SNARK; SNF1/AMP activated protein kinase
• Gene ID: 81788
• mRNA Refseq: NM_030952
• Protein Refseq: NP_112214
• MIM: 608131
• UniProt ID: Q9H093
• Chromosome Location: 1q32.1
• Function: ATP binding; metal ion binding; protein binding; protein serine/threonine kinase activity