| Description : |
TNFSF10 is a member of thetumor necrosis factor family. TNFSF10 functions as aligandwhich induces the process of cell death calledapoptosis. TNFSF10 shows homology to other members of thetumor necrosis factorsuperfamily. It is composed of 281 amino acids (aa) and has characteristics of a type IItransmembrane protein. The N-terminal cytoplasmic domain is not conserved across family members, however, the C-terminal extracellular domain is conserved and can be proteolytically cleaved from the cell surface. |
| Species : |
Human |
| Enzyme Unit : |
10,000 International units (IU). The assigned potency is in International Units (IU) that reflect results derived from cytotoxicity assays. These IU are specific to TRAIL and are not continuous with the IU of TNF-alpha or TNF-beta. |
| Usage : |
No attempt should be made to weigh out any portion of the freeze-dried material prior to reconstitution. For all practical purposes each ampoule contains the same quantity of the substances listed above. Dissolve the total contents of the ampoule with 0.5ml of sterile distilled water. Rinse the ampoule with 0.4ml of sterile distilled water and make up the total volume to 1ml with sterile distilled water. This solution should now be at a concentration of 10,000 IU/ml. Use carrier protein where extensive dilution is required. For economy of use, it is recommended that the solution be sub-divided into several small aliquots and stored at -40 °C or below. |
| Notes : |
This preparation is not for administration to humans. The preparation contains human and bovine source material. The preparation contains an excipient of human origin which has been tested and found negative for HBsAg and HIV antibody. The preparation has subsequently been tested and found negative for anti- HCV and HCV RNA by PCR. The bovine casein used as an excipient was sourced from a country where bovine spongiform encephalopathy (BSE) has not been found. As with all materials of biological origin, this preparation should be regarded as potentially hazardous to health. It should be used and discarded according to your own laboratorys safety procedures. Such safety procedures should include the wearing of protective gloves and avoiding the generation of aerosols. Care should be exercised in opening ampoules or vials, to avoid cuts. |
| Stability : |
Reference materials are held at Creative Biomart within assured, temperature- controlled storage facilities and they should be stored on receipt as indicated on the label. Accelerated degradation studies have indicated that this material is suitably stable, when stored at -20°C or below, for the assigned values to remain valid until the material is withdrawn or replaced. These studies have also shown that the material is suitably stable for shipment at ambient temperature without any effect on the assigned values. Once reconstituted, diluted or aliquoted, users should determine the stability of the material according to their own method of preparation, storage and use. Users who have data supporting any deterioration in the characteristics of any reference preparation are encouraged to contact Creative Biomart. |
| Storage : |
Unopened ampoules should be stored at -20 °C . Please note: because of the inherent stability of lyophilized material, Creative Biomart may ship these materials at ambient temperature. |
