Recombinant Human Platelet-derived Growth Factor-BB (rDNA derived) Reference standard
Cat.No. : | PDGFBB-141H |
Product Overview : | The PDGF-BB used in this preparation is the human form of the molecule, synthesized in E. coli by recombinant DNA technology. |
- Specification
- Gene Information
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Source : | E. coli |
Species : | Human |
Enzyme Unit : | The assigned potency PDGF-BB is 3000 International Units (IU) per ampoule. |
Usage : | No attempt should be made to weigh out any portion of the freeze-dried material prior to reconstitution. The reference material is intended for calibration of local standards. For all practical purposes, each ampoule contains the same quantity of PDGF-BB. The entire contents of each ampoule should be completely dissolved in a known volume of suitable solvent. It is recommended that, when possible, buffer containing carrier protein should be used to minimize loss by surface adsorption. |
Notes : | This preparation is not for administration to humans. The preparation contains material of human origin, and either the final product or the source materials, from which it is derived, have been tested and found negative for HBsAg, anti-HIV and HCV RNA. As with all materials of biological origin, this preparation should be regarded as potentially hazardous to health. It should be used and discarded according to your own laboratorys safety procedures. Such safety procedures should include the wearing of protective gloves and avoiding the generation of aerosols. Care should be exercised in opening ampoules or vials, to avoid cuts. |
Stability : | .Reference materials are held at Creative Biomart within assured, temperature- controlled storage facilities and they should be stored on receipt as indicated on the label. Accelerated degradation studies have indicated that this material is suitably stable, when stored at -20°C or below, for the assigned values to remain valid until the material is withdrawn or replaced. These studies have also shown that the material is suitably stable for shipment at ambient temperature without any effect on the assigned values. Users who have data supporting any deterioration in the characteristics of any reference preparation are encouraged to contact Creative Biomart. |
Storage : | The ampoules are shipped at ambient temperature. Unopened ampoules should be stored at -20 °C in the dark. For economy of use, it is recommended that the reconstituted solution be subdivided into several small containers and stored at, or below, -40 °C . Repeated freezing and thawing should be avoided. The ampoules do not contain bacteriostat and solutions of the ampouled material should not be assumed to be sterile. Please note: because of the inherent stability of lyophilized material, Creative Biomart may ship these materials at ambient temperature. |
Gene Name : | PDGFB platelet-derived growth factor beta polypeptide [ Homo sapiens ]; |
Official Symbol : | PDGFB |
Synonyms : | SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2; platelet-derived growth factor subunit B; becaplermin; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) |
Gene ID : | 5155 |
mRNA Refseq : | NM_002608 |
Protein Refseq : | NP_002599 |
MIM : | 190040 |
UniProt ID : | P01127 |
Chromosome Location : | 22q13.1 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; DAP12 signaling, organism-specific biosystem |
Function : | chemoattractant activity; collagen binding; identical protein binding |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionBy potently stimulating the migratory and proliferative capabilities of smooth muscle cells and pericytes, PDGFB plays a pivotal role in regulating angiogenesis and orchestrating vascular remodeling events, thereby ensuring proper tissue perfusion and function.
PDGFB signaling assumes a critical role in fueling tumor growth, angiogenesis, and metastasis. PDGFB, acting through its activation of PDGFRβ, elicits a pro-tumorigenic cascade that promotes tumor cell proliferation, survival, migration, and invasion, while concurrently fueling tumor vascularization and distant metastatic spread.
PDGFB emerges as a significant contributor to the pathogenesis of fibrotic diseases by prompting fibroblast activation and proliferation. These dysregulated processes lead to excessive extracellular matrix deposition and subsequent tissue scarring. Furthermore, PDGFB exacerbates fibrosis through induction of myofibroblast differentiation.
Promisingly, preclinical and clinical studies have investigated diverse approaches targeting the PDGFB/PDGFRβ signaling axis for therapeutic intervention in fibrotic disorders and certain neoplastic conditions. Inhibitors directed against PDGFB or PDGFRβ have exhibited considerable potential, effectively blocking downstream signaling cascades activated by PDGFB, thereby hampering cell proliferation and angiogenesis.
PDGFB, released by platelets and macrophages at inflammatory sites, acts as a key modulator in recruiting and activating fibroblasts. This activation not only promotes collagen synthesis but also initiates tissue remodeling, thus expediting the wound healing cascade and tissue integrity restoration.
Several therapeutic strategies aim to target PDGFB for disease treatment. Inhibitors of PDGFB, such as tyrosine kinase inhibitors, have been developed and used in clinical settings. These inhibitors can selectively block the activation of PDGF receptors and inhibit downstream signaling pathways, thereby suppressing excessive cell proliferation and migration. Additionally, approaches targeting PDGFB expression, such as gene therapy or RNA interference, hold promise as potential treatments for diseases associated with PDGFB dysregulation.
PDGFB orchestrates cellular proliferative responses and drives growth by virtue of its ability to activate PDGFRβ, which in turn triggers downstream signaling cascades facilitating DNA synthesis and regulating critical cell cycle checkpoints.
Customer Reviews (3)
Write a reviewThe seal of the reagent tubes is excellent, preventing evaporation and leakage.
With its unique design and construction, the experimental reagent provides me with a fresh experimental experience.
The stability of this reagent is exceptional, ensuring the reliability of the experiment.
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