Recombinant Human ISL1

• Cat.No.: ISL1-129H
• Product Overview: Recombinant human ISL1 protein fragment (AA 1-100) was expressed in E. coli and refolded to soluble form.

Specification

• Description: ISL1 is a transcription factor that has been shown to bind a cis-acting domain of the insulin gene enhancer. It contains two N-terminal LIM zinc-binding domains, and one homeobox DNA-binding domain. During embryonic development, ISL1 has been identified as a marker for cardiovascular progenitor cells that form the three major cell types in the heart (cardiac, smooth muscle, and endothelial cells). In addition, ISL1 is a marker for endocrine progenitor cells in the pancreas, and plays a critical role in the development of mature islet beta cells.
• Source: E. coli
• Species: Human
• Form: 1.4 mg/ml (determined by Bradford assay) in hs-PBS (1.5 mM KH2PO4, 8.1 mM Na2HPO4, 500 mM NaCl, 2.7 mM KCl, pH 7.4).
• Molecular Mass: 11.5 kDa (AA 1-100).
• Purity: >90% by SDS-PAGE
• Storage: Store at -80 °C. Avoid multiple freeze-thaws.

Gene Information

• Gene Name: ISL1 ISL LIM homeobox 1 [ Homo sapiens ]
• Official Symbol: ISL1
• Synonyms: ISL1; ISL LIM homeobox 1; ISL1 transcription factor, LIM/homeodomain, (islet 1); insulin gene enhancer protein ISL-1; Isl 1; ISLET1; islet-1; ISL1 transcription factor, LIM/homeodomain; Isl-1
• Gene ID: 3670
• mRNA Refseq: NM_002202
• Protein Refseq: NP_002193
• MIM: 600366
• UniProt ID: P61371
• Chromosome Location: 5q11.2
• Pathway: Heart Development, organism-specific biosystem; Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Regulation of Insulin Secretion, organism-specific biosystem; Synthesis, Secretion, and Inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP), organism-specific biosystem
• Function: RNA polymerase II activating transcription factor binding; RNA polymerase II transcription coactivator activity; bHLH transcription factor binding; chromatin binding; enhancer sequence-specific DNA binding; enhancer sequence-specific DNA binding; estrogen receptor binding; ligand-dependent nuclear receptor binding; metal ion binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; zinc ion binding