||Collagen from washed dissected tissue was dissolved in acetic acid after mild pepsin treatment. Collagen type I was then purified using differential salt precipitation.
||Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis.
||Greater than 90% as determined by SDS-PAGE.
||This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
||Store at -20 °C only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.If stored in this manner, this product is stable for 18 months after receipt.
||Use 0.05-0.5 M acetic acid, pH 2.5 at 4°C.