Recombinant Human HUWE1, GST-tagged

• Cat.No.: HUWE1-136H
• Product Overview: Recombinant human HUWE1 (amino acid residues 3760-4374), with N-terminal GST, was expressed in E.coli.

Specification

• Description: The enzymes of the ubiquitylation pathway play a pivotal role in a number of cellular processes including the regulated and targeted proteasome-dependent degradation of substrate proteins. Three classes of enzymes are involved in the process of ubiquitylation; activating enzymes (E1s), conjugating enzymes (E2s) and protein ligases (E3s). Hect, Uba, and Wwe Domains containing protein 1 (HUWE1) is a member of the Homologous to the E6AP Carboxyl Terminus (HECT) E3 protein ligase family and cloning of the human gene was first described by (Liu et al., 2005).
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Molecular Mass: ~97kDa
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5mg/ml

Gene Information

• Gene Name: HUWE1 HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase [ Homo sapiens ]
• Official Symbol: HUWE1
• Synonyms: HUWE1; HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase; HECT, UBA and WWE domain containing 1; E3 ubiquitin-protein ligase HUWE1; Ib772; KIAA0312; UREB1; ARF-binding protein 1; URE-binding protein 1; BJ-HCC-24 tumor antigen; large structure of UREB1; HECT domain protein LASU1; Mcl-1 ubiquitin ligase E3; upstream regulatory element-binding protein 1; homologous to E6AP carboxyl terminus homologous protein 9; MULE; LASU1; HECTH9; URE-B1; ARF-BP1; HSPC272
• Gene ID: 10075
• mRNA Refseq: NM_031407
• Protein Refseq: NP_113584
• MIM: 300697
• UniProt ID: Q7Z6Z7
• Chromosome Location: Xp11.22
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem
• Function: DNA binding; acid-amino acid ligase activity; binding; ligase activity; protein binding; ubiquitin-protein ligase activity