"PPK" Related Products

Recombinant Polyphosphate Kinase

Cat. No.: PPK-85H
Product Overview: Recombinant Human Polyphosphate Kinase purified circa 10 fold. Free of all Polyphosphate Glucokinase activity. Native MW = 83 kD (monomer)
Description: PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.
Synonyms: EC 2.7.4.1;Polyphosphate kinase; Polyphosphoric acid kinase; ATP-polyphosphate phosphotransferase; ATP:polyphosphate phosphotransferase.
Source: Propionibacterium shermanii.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized from PPK solution containing 11.54 U/ml of PPK activity, 10.3 mg/ml total protein, 100 mM potassium phosphate pH 6.8 and 25 mM sodium polyphosphate.
Specific Activity: 1.12 U/mg.
Definition of Activity Units: The amount of Polyphosphate kinase required to convert 1 µmole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor.
Required Cofactors: The reaction requires Mg2+.
Solubility: The contents of the vial can be reconstituted in 1-10 ml deionized water.
Stability: Lyophilized Polyphosphate kinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PPK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Products Types

◆ Native Protein

Related Gene
PPK

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