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Active Recombinant Human IL23 (IL12B & IL23A Heterodimer), His-tagged

Cat.No. : IL23A&IL12B-208H
Product Overview : A DNA sequence encoding the IL23A (Q9NPF7) (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL23 heterodimer was purified.
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Description : This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.
Source : Human Cells
Species : Human
Tag : His
Predicted N Terminal : Arg 20 & Ile 23
Form : Lyophilized from sterile PBS, pH 7.4
Bio-activity : 1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA.2. Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 μg/ml.3.Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.14-0.35 μg/ml.
Molecular Mass : The recombinant IL23 heterodimer of human IL23A/IL12B comprises 498 (181 + 317) amino acids and has a calculated molecular mass of 56.3 (20.1 + 36.2) kDa. The apparent molecular mass of rhIL23 heterodimer is approximately 22 & 45 kDa respectively in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -70°C
Storage : Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : A hardcopy of COA with reconstitution instruction is sent along with the products.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Publication :
Cytokine and reactivity profiles in SLE patients following anti-CD19 CART therapy (2023)
Gene Name : IL23A interleukin 23, alpha subunit p19 [ Homo sapiens (human) ]
Official Symbol : IL23A
Synonyms : IL23A; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; IL 23; IL 23A; IL23P19; interleukin six; G CSF related factor; P19; SGRF
Gene ID : 51561
mRNA Refseq : NM_016584
Protein Refseq : NP_057668
MIM : 605580
UniProt ID : Q9NPF7
Chromosome Location : 12q13.13
Pathway : Cytokine-cytokine receptor interaction; IL23-mediated signaling events; Inflammatory bowel disease (IBD)
Function : cytokine activity; contributes_to interleukin-23 receptor binding; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Does IL23 expression correlate with disease severity in psoriasis? 05/12/2022

IL23 expression correlates with disease severity in psoriasis.

Does IL23 expression vary between different immune cell types? 06/03/2021

IL23 expression varies among dendritic cells and macrophages.

What are the downstream targets of IL23 signaling? 07/24/2020

IL23 activates STAT3 to modulate downstream targets.

Does IL23 interact with other cytokines in a signaling cascade? 06/07/2019

IL23 interacts with IL-12 to form a signaling cascade.

Is IL23 involved in the activation of STAT3? 04/29/2019

IL23 is involved in the activation of STAT3.

How does IL23 expression change in autoimmune diseases? 10/02/2018

IL23 expression is upregulated in autoimmune diseases like Crohn's.

What is the half-life of IL23 in plasma? 01/14/2018

The half-life of IL23 in plasma is approximately 2 hours.

How does IL23 affect macrophage polarization? 10/25/2017

IL23 induces M1 macrophage polarization.

What is the role of IL23 in Th17 cell differentiation? 03/19/2017

IL23 promotes Th17 cell differentiation by activating STAT3.

How does IL23 contribute to tissue inflammation? 01/27/2016

IL23 contributes to tissue inflammation by activating macrophages.

Customer Reviews (3)

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Reviews
12/17/2021

    High purity levels in CHIP

    12/26/2018

      Effective in T-cell differentiation.

      09/25/2018

        Suitable for signaling studies.

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