||Recombinant human eIF5A protein was expressed in E. coli and purified by using conventional chromatography techniques, 16.8 kDa (154 aa).
||Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.
||Liquid. In 50 mM Tris-HCl buffer pH 7.5 containing 10 % glycerol
||> 95% by SDS - PAGE.
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQ DG YL S LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.