||Recombinant extracellular Ig-like domain of NKp46( 22-255aa) was overexpressed in E.coli, and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer (235 aa, 26.6 kDa).
||A natural cytotoxicity receptor(NCR) NKp46 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. The natural cytotoxicity receptors(NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. The three known NCRs include NKp46 and NKp30,which are expressed on circulating NKcells, and NKp44,which is expressed only on activating NK cells. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells and pathogen-infected cell lines. NKp46 has two extracellular Ig-like domains followed by a ~40 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail.
||Liquid. In phosphate buffered saline (pH 7.4) containing 1 mM EDTA.
||26.6 kDa (235 aa).
||> 95% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||MQQQTLPKPF IWAEPHFMVP KEKQVTICCQ GNYGAVEYQL HFEGSLFAVD RPKPPERINK VKFYIPDMNS RMAGQYSCIY RVGELWSEPS NLLDLVVTEM YDTPTLSVHP GPEVISGEEV TFYCRLDTAT SMFLLLKEGR SSHVQRGYGK VQAEFPLGPV TTAHRGTYRX FGSYNNHAWS FPSEPVKLLV TGDIENTSLA PEDPTFSADT WGTYLLTTET GLQKDHALWD HTAQN
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.