||Recombinant human MYL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 20.9 kDa (186aa)
||Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MAPKKAKKRA GGANSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGVLKADYVR EMLTTQAERF SKEEVDQMFA AFPPDVTGNL DYKNLVHIIT HGEEKD
||Liquid in 20mM Tris-HCl (pH 8.0) containing 20% glycerol 5mM CaCl2
||> 95% by SDS PAGE
||0.5 mg/ml (determined by Bradford assay)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.