Active Recombinant Human Tumor Necrosis Factor

• Cat.No.: TGFA-7H
• Product Overview: Human TNF- alpha (Cachectin) is produced in genetically engineered yeast, and purified by sequential chromatography.

Specification

• Description: Human Tumor necrosis factor (TNF- alpha), also known as Cachectin, is a cytokine composed of 157 amino acid residues, having a molecular weight of 17 kDa (monomer). There is 79% homology between human and murine TNF- alpha, including conserved cysteine residues which form an intrachain disulfide bridge. Biological effects of this cytokine include antitumor activity in vivo and in vitro, activation of polymorphonuclear leukocytes, antiviral activity, and the induction of the release if Interleukin-1 or colony stimulating factors from a variety of sources.
• Source: yeast
• Species: Human
• Form: At concentration of 1 mg/ml in 0.02M Tris-HCl/ 0.15 M NaCl (pH 8.0)
• Bio-activity: The cell cytolytic activity is determined in the L929 cells (3 x 104 cells/assay) and it is 2-4 x 108 U/mg.
• Molecular Mass: 17 KDa
• Purity: Over 95% pure by SDS gel electrophoresis.
• Stability: At least 1 year at -80°C.
• Storage: Store at -80°C.

Gene Information

• Gene Name: TGFA?transforming growth factor, alpha [?Homo sapiens?(human) ]
• Official Symbol: TGFA
• Synonyms: TGFA; transforming growth factor, alpha; TFGA; protransforming growth factor alpha; transforming growth factor-alpha; TGF-alpha; EGF-like TGF; TGF type 1; ETGF
• Gene ID: 7039
• mRNA Refseq: NM_001099691
• Protein Refseq: NP_001093161
• MIM: 190170
• UniProt ID: P01135
• Chromosome Location: 2p13
• Pathway: ErbB signaling pathway; Glioma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Renal cell carcinoma
• Function: MAP kinase kinase activity; epidermal growth factor receptor binding; growth factor activity; protein binding; signal transducer activity