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Recombinant Human Fas Ligand soluble, FLAG-tagged

Cat.No. : FAS-38H
Product Overview : The extracellular domain of human FasL (sFasL, aa. 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG-tag. Glycosylation of rhsFasL is similar to natural human FasL. The recombinant protein is produced in HEK 293 cells.
  • Specification
  • Gene Information
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Source : HEK 293 cells
Species : Human
Tag : FLAG
Form : Provided as a lyophilized powder. Contains PBS.
Molecular Mass : ~32 kDa (non-glycosylated), ~35 kDa (glycosylated) (SDS-PAGE)
Endotoxin : Endotoxin content is<0.1 eu/μg purified protein as determined by lal test.>
Purity : ≥95% purity as determined by SDS-PAGE.
Characteristic : rhsFasL binds to human, mouse and rat Fas.
Applications : Induction of Apoptosis: Recombinant FasL protein induces apoptosis in Fas-sensitive cells (ED50: 50 ng/mL in A20 cells). Optimal concentration varies with cell type and should be determined by testing serial dilutions on cells. Alone, rhsFasL kills Fas-sensitive cells at a concentration of >10 ng/mL. Use of a secondary enhancing cross-linking antibody reacting with rhsFasL increases the activity of rhsFasL approximately 50-fold.
Usage : For in vitro research use only. Not for use in diagnostics or in humans.
Notes : Results using sFasL may differ from those obtained with agonistic antibodies.
Storage : Store at -20°C long term. Once rehydrated it is recommended to prepare appropriate aliquots and store them at -20°C. Avoid repeated freeze/thaw cycles.
Reconstitution : Prepare sterile stock solution by dissolving rhsFasL in 100 μL sterile water (0.1 mg/mL in PBS). Further dilutions should be made with medium containing 5% fetal calf serum.
Warning : No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.
Gene Name : FAS Fas (TNF receptor superfamily, member 6) [ Homo sapiens ]
Official Symbol : FAS
Synonyms : FAS; Fas (TNF receptor superfamily, member 6); APT1, FAS1, TNFRSF6, tumor necrosis factor receptor superfamily, member 6; tumor necrosis factor receptor superfamily member 6; APO 1; CD95; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; APO-1 cell surface antigen; apoptosis-mediating surface antigen FAS; tumor necrosis factor receptor superfamily, member 6; APT1; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6;
Gene ID : 355
mRNA Refseq : NM_000043
Protein Refseq : NP_000034
MIM : 134637
UniProt ID : P25445
Chromosome Location : 10q24.1
Pathway : Activation of Pro-Caspase 8, organism-specific biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Alzheimers disease, organism-specific biosystem;
Function : binding; identical protein binding; kinase binding; protein binding; receptor activity; receptor activity; signal transducer activity; transmembrane signaling receptor activity; tumor necrosis factor-activated receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Does FAS protein play a role in cell signaling? 10/30/2022

FAS protein plays an important role in cell signaling, it can activate downstream signaling pathways, such as death signaling pathway and survival signaling pathway, by binding to its ligand and transmitting signals.

Why apply knowledge of FAS proteins to clinical practice? 06/27/2022

It can provide a reference for clinical practice and guide the diagnosis, treatment and prevention of diseases by detecting the expression level of FAS protein and understanding its mechanism of action.

How can diseases associated with FAS protein be prevented and treated? 05/13/2022

FAS protein-related diseases can be prevented and treated by regulating the expression level of FAS protein and inhibiting its signaling pathway.

How can gene knockout or knockdown techniques be used to study the function of FAS proteins? 03/31/2021

Gene knockout or knockdown techniques can be used to study the function and mechanism of action of FAS proteins. By knocking out or knocking down the FAS gene, changes in processes such as apoptosis and immune response can be observed to further understand its function and mechanism of action.

What is the role of FAS protein in apoptosis? 09/29/2020

FAS protein can bind to its ligand, activate the death signaling pathway, and induce apoptosis.

How can FAS protein be used to predict disease prognosis? 06/19/2020

The expression level and activity of FAS protein can be measured to predict the prognosis and development trend of certain diseases.

Customer Reviews (3)

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Reviews
09/07/2022

    When experimenting with FAS, the results are very reproducible.

    07/29/2022

      The stability of FAS products allows them to cope with different experimental conditions and modes of operation.

      09/20/2021

        FAS has high specific activity and can exert strong biological effect at low concentration.

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