Active Recombinant Human PARP-1

• Cat.No.: PARP1-58H
• Product Overview: Recombinant protein produced in insect cells (Sf9).

Specification

• Source: Sf9 cells
• Species: Human
• Form: 20 μg at 1 mg/mL affinity-purified liquid human recombinant PARP-1 in 100 mM Tris-HCl (pH 7.5) containing 14 mM β-mercaptoethanol, 0.5 mM EDTA, 0.5 mM PMSF and 10% glycerol.
• Bio-activity: ~1,018 U/mg protein.
• Purity: >99% as determined by SDS-PAGE.
• Unit Definition: One unit synthesizes 1 nmole of poly(ADP-ribose) per min. at 25°C, pH 7.5.
• Usage: For in vitro research use only. Not for use in diagnostics or in humans.
• Storage: Store at -80°C. Aliquot to avoid repeated freeze/thaw cycles.
• Warning: No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.

Gene Information

• Gene Name: PARP1 poly (ADP-ribose) polymerase 1 [ Homo sapiens ]
• Official Symbol: PARP1
• Synonyms: PARP1; poly (ADP-ribose) polymerase 1; ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) , ADPRT, poly (ADP ribose) polymerase family, member 1 , PPOL; poly [ADP-ribose] polymerase 1; PARP; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); PPOL; ADPRT; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
• Gene ID: 142
• mRNA Refseq: NM_001618
• Protein Refseq: NP_001609
• MIM: 173870
• UniProt ID: P09874
• Chromosome Location: 1q41-q42
• Pathway: BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Caspase cascade in apoptosis, organism-specific biosystem; FAS pathway and Stress induction of HSP regulation, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem
• Function: DNA binding; NAD binding; NAD+ ADP-ribosyltransferase activity; metal ion binding; protein N-terminus binding; protein binding; transcription factor binding; transferase activity, transferring glycosyl groups; zinc ion binding