||Recombinant TCEB2 was expressed as insoluble protein aggregate in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer, 13.1 kDa (118 aa).
||Elongin B , also known as TCEB2, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimer consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C). It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation.
||Liquid. In Phosphate buffered saline (pH7.4), 10% glycerol
||> 95% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MEGCVSNLMV CNLAYSGKLE ELKESILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLGKGAQVN AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYEA TAMHRAAAKG NLKMIHILLY YKASTNIQDT EGNTPLHLAC DEERVEEAKL LVSQGASIYI ENKEEKTPLQ VAKGGLGLIL KRMVEG
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.