||Recombinant human UBE2L6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 21.7kDa (188aa).
||Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene.
||Liquid in 20mM Tris pH 8.0, 1 mM DTT, 0.1 mM PMSF 10% glycerol.
||> 90% by SDS PAGE.
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASM RVVKE LEDLQ KKPPPYLRNL SSDDANVLVW HALLLPDQPP YHLKAFNLRI SFP PEYPFKP PMIKFTTKIY HPNVDENGQI CLPIISSENW KPCTKTCQVL EALN VLVNRP NIREPLRMDL ADLLTQNPEL FRKNAEEFTL RFGVDRPS
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.