||Recombinant human VEGF-B protein was expressed in E.coli and purified by using conventional chromatography techniques, 21.6 kDa (207 aa).
||VEGF-B, also known as Vascular endothelial growth factor-B, is one of 5 mammalian VEGF family members, the others being VEGF-A, VEGF-C, VEGF-D, and placental growth factor (PlGF). VEGF-B binds to one of the VEGF receptor tyrosine kinases, namely VEGF receptor-1(VEGFR1, Flt1), but not to VEGFR2 and VEGFR3. VEGF-B is homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis.
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA
||Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol
||> 90% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.