Recombinant Human Tyrosine 3-monooxygenase/ Tryptophan 5-monooxygenase Activation Protein, Zeta Polypeptide, His-tagged

• Cat.No.: YWHAZ-104H
• Product Overview: Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (245 aa, 32kDa).

Specification

• Cat. No.: YWHAZ-104H
• Description: The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ and η that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity.
• Source: E.coli.
• Formulation: Liquid in Phosphate Buffered Saline pH7.4.
• Molecular Weight: 32 kDa (245 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publication:
  14-3-3ζ protein protects against brain ischemia/reperfusion injury and induces BDNF transcription after MCAO in rat (2019)

Gene Information

• Gene Name: YWHAZ
• Synonyms: YWHAZ; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; KCIP-1; MGC111427; MGC126532; MGC138156; phospholipase A2; 14-3-3 zeta; 14-3-3 protein/cytosolic phospholipase A2; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; OTTHUMP00000165851; OTTHUMP00000165852; OTTHUMP00000165854; OTTHUMP00000165858; OTTHUMP00000165859; OTTHUMP00000165860; YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide.
• Gene ID: 7534
• mRNA Refseq: NM_001135699.1
• Protein Refseq: NP_001129171.1
• MIM: 601288
• UniProt ID: P63104
• Chromosome Location: 8q23.1
• Pathway: Cell cycle; Pathogenic Escherichia coli infection - EHEC; Pathogenic Escherichia coli infection - EPEC.
• Function: protein domain specific binding; transcription factor binding.