Active Recombinant Human ALK Protein, GST-tagged

• Cat.No.: ALK-01H
• Product Overview: Recombinant human ALK (L1196M G1202R) (1060-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).
• Source: Insect Cell
• Species: Human
• Tag: GST
• Bio-activity: 18 nmol/min/mg
• Molecular Mass: 90 kDa
• Purity: > 80%
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Gene Information

• Gene Name: ALK ALK receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: ALK
• Synonyms: ALK; ALK receptor tyrosine kinase; CD246; NBLST3; ALK tyrosine kinase receptor; CD246 antigen; anaplastic lymphoma receptor tyrosine kinase; mutant anaplastic lymphoma kinase; EC 2.7.10.1
• Gene ID: 238
• mRNA Refseq: NM_004304
• Protein Refseq: NP_004295
• MIM: 105590
• UniProt ID: Q9UM73