Recombinant Human RAC1(Q61L), His-tagged

• Cat.No.: RAC1-191H
• Product Overview: Recombinant Human RAC1(Q61L), fused with N-terminus His-tagged, was expressed in a bacterial system. The leucine substitution prevents endogenous and GAP-stimulated GTPase activity of Rac1, hence the protein is always in the active, GTP-bound, state.

Specification

• Description: The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Bacterial
• Species: Human
• Tag: His
• Form: The protein is supplied as a lyophilized powder.
• Molecular Mass: The recombinant protein is 25 kDa, consisting of the 22 kDa Rac1 constitutively active protein plus a histidine tag in the amino-terminus.
• Purity: >90% pure by SDS-PAGE
• Storage: The protein must not be exposed to repeated freeze-thaw cycles. The lyophilized protein is stable at 4ºC desiccated (<10% humidity) for six months.
• Reconstitution: When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose and 0.1% dextran.

Gene Information

• Gene Name: RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) [ Homo sapiens (human) ]
• Official Symbol: RAC1
• Synonyms: RAC1; MGC111543; MIG5; TC-25; PAK1; TC25; P21-RAC1; RAC-1; p21-Rac1;Ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); RAS-like protein; Ras-related C3 botulinum toxin substrate 1; rho family; small GTP binding protein Rac1; Ras-related C3 botulinum toxin substrate 1; Cell migration-inducing gene 5 protein; Ras-like protein TC25; migration-inducing gene 5; migration-inducing protein 5; ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); ras-related C3 botulinum toxin substrate 1 isoform Rac1; ras-related C3 botulinum toxin substrate 1 isoform Rac1b; rho family, small GTP binding protein Rac1
• Gene ID: 5879
• mRNA Refseq: NM_006908
• Protein Refseq: NP_008839
• MIM: 602048
• UniProt ID: P63000
• Chromosome Location: 7p22
• Pathway: Adherens junction; Amyotrophic lateral sclerosis (ALS); Androgen receptor signaling pathway
• Function: GTP binding; GTP-dependent protein binding; Rho GDP-dissociation inhibitor binding