Recombinant P. heparinus Heparinase III, Met&His-tagged Heparinase III-205P

Recombinant P. heparinus Heparinase III, Met&His-tagged

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Recombinant P. heparinus Heparinase III, Met&His-tagged

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Cat.No. : Heparinase III-205P
Product Overview : Recombinant P. heparinus Heparinase III (Accession # AAB18278) Gln25-Pro659, fused with an N-terminal Met and 6-His tag, was produced in E. coli-derived.
Source : E. coli
Species : P. heparinus
Tag : &His
Predicted N Terminal : Met
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
Bio-activity : Measured by its ability to liberate oligosaccharides from heparin.The specific activity is >3,000 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
Molecular Mass : Recombinant P. heparinus Heparinase III, Met&His-tagged has a calculated MW of 74 kDa. In SDS-PAGE migrates as 66 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

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