Active Recombinant P. heparinus Heparinase III Protein, His-tagged

• Cat.No.: Heparinase III-205P
• Product Overview: Recombinant P. heparinus Heparinase III Protein Gln25-Pro659, with an N-terminal Met and 6-His tag was expressed in E. coli.

Specification

• Description: Heparan sulfate is a sulfated glycosaminoglycan with the repeating disaccharide units of -4HexA1,4GlcNAc beta 1. It is usually attached to the protein cores of proteoglycans found on the cell membrane and in the extracellular matrix where it binds to a variety of protein ligands and regulates a wide range of biological activities. Heparan sulfate has a domain structure where sulfated regions are interspaced with less or non-sulfated regions. Heparin shares the backbone structure with heparan sulfate but contains no non‑sulfated regions. Heparinases are a family of lyases that release unsaturated oligosaccharides from heparin and heparan sulfate upon digestion. Heparinase I recognizes highly sulfated regions and is more specific for heparin. Heparinase II digests both heparin and heparan sulfate. Heparinase III prefers less-sulfated regions and is more active on heparan sulfate.
• Source: E. coli
• Species: P. heparinus
• Tag: His
• Bio-activity: Measured by its ability to liberate oligosaccharides from heparin. The specific activity is >3,000 pmol/min/μg.
• Molecular Mass: Predicted Molecular Mass: 74 kDa
  SDS-PAGE: 66 kDa, reducing conditions
• N-terminal Sequence Analysis: Met
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
• Applications: Enzyme Activity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Shipping: The product is shipped with polar packs.