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Recombinant Human PLA2G7, His-tagged

Cat.No.: PLA2G7-329H
Product Overview: Recombinant Human PLA2G7 (Accession # AAH38452) Met33-Asn441 & Leu45-Asn441, fused with a C-terminal 6-His tag, was produced in E. coli-derived.
Source: E. coli
Species: Human
Tag: His
Predicted N Terminal: Met33 & Leu45
Form: Supplied as a 0.2 µm filtered solution in Sodium Acetate, NaCl and Glycerol.
Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, 1-O-hexadecyl-2-deoxy-2-thio-S-acetyl-sn-glyceryl-3-phosphorylcholine (2-Thio-PAF), in the presence of 5,5"Dithio-bis(2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. Stewart, A.G. et al. (1991) J. Lipid Mediat 4:299. Aarsman, A.J. et al. (1991) Eur. J. Biochem 200:187.The specific activity is >9,000 pmol/min/ug, as measured under the described condition.
Molecular Mass: Recombinant Human PLA2G7, His-tagged has a calculated MW of 46 kDa. In SDS-PAGE migrates as 42 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: PLA2G7 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) [ Homo sapiens ]
Official Symbol: PLA2G7
Synonyms: PLA2G7; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); platelet-activating factor acetylhydrolase; LDL PLA2; PAFAH; LDL-PLA(2); gVIIA-PLA2; PAF 2-acylhydrolase; PAF acetylhydrolase; group-VIIA phospholipase A2; LDL-associated phospholipase A2; lipoprotein-associated phospholipase A2; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; PAFAD; LP-PLA2; LDL-PLA2;
Gene ID: 7941
mRNA Refseq: NM_001168357
Protein Refseq: NP_001161829
MIM: 601690
UniProt ID: Q13093
Chromosome Location: 6p21.2-p12

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