Active Recombinant Human PLA2G7, His-tagged

• Cat.No.: PLA2G7-329H
• Product Overview: Recombinant Human PLA2G7 (Accession # AAH38452) Met33-Asn441 & Leu45-Asn441, fused with a C-terminal 6-His tag, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met33 & Leu45
• Form: Supplied as a 0.2 µm filtered solution in Sodium Acetate, NaCl and Glycerol.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, 1-O-hexadecyl-2-deoxy-2-thio-S-acetyl-sn-glyceryl-3-phosphorylcholine (2-Thio-PAF), in the presence of 5,5"Dithio-bis(2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. Stewart, A.G. et al. (1991) J. Lipid Mediat 4:299. Aarsman, A.J. et al. (1991) Eur. J. Biochem 200:187.The specific activity is >9,000 pmol/min/ug, as measured under the described condition.
• Molecular Mass: Recombinant Human PLA2G7, His-tagged has a calculated MW of 46 kDa. In SDS-PAGE migrates as 42 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PLA2G7 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) [ Homo sapiens ]
• Official Symbol: PLA2G7
• Synonyms: PLA2G7; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); platelet-activating factor acetylhydrolase; LDL PLA2; PAFAH; LDL-PLA(2); gVIIA-PLA2; PAF 2-acylhydrolase; PAF acetylhydrolase; group-VIIA phospholipase A2; LDL-associated phospholipase A2; lipoprotein-associated phospholipase A2; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; PAFAD; LP-PLA2; LDL-PLA2
• Gene ID: 7941
• mRNA Refseq: NM_001168357
• Protein Refseq: NP_001161829
• MIM: 601690
• UniProt ID: Q13093
• Chromosome Location: 6p21.2-p12