Active Recombinant Human IFNG, Met-tagged

• Cat.No.: IFNG-473H
• Product Overview: Recombinant Human IFNG (Accession # CAA31639) Gln24-Gln166, fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
• Bio-activity: Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.The ED50 for this effect is typically 0.15-0.75 ng/mL.
• Molecular Mass: Recombinant Human IFNG, Met-tagged has a calculated MW of 16.9 kDa. In SDS-PAGE migrates as 17 kDa, reducing conditions.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile, deionized water.

Gene Information

• Gene Name: IFNG interferon, gamma [ Homo sapiens ]
• Official Symbol: IFNG
• Synonyms: IFNG; interferon, gamma; interferon gamma; IFN-gamma; immune interferon; IFG; IFI
• Gene ID: 3458
• mRNA Refseq: NM_000619
• Protein Refseq: NP_000610
• MIM: 147570
• UniProt ID: P01579
• Chromosome Location: 12q14