Recombinant Human IL6

Cat.No.: IL6-2778H
Product Overview: Recombinant human IL-6 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 184 amino acids.
Description: Interleukin 6 (IL-6) is a pleiotropic α helical cytokine that plays important roles in acute phasereactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a phosphorylated and variably glycosylated molecule. Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Human IL-6 is equally active on mouse and rat cells. IL6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6R, triggering IL-6R association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT1, IL-11,IL-27, LIF, and OSM. Soluble forms of IL-6R are generated by both alternate splicing and proteolytic cleavage . In a mechanism known as transsignaling, complexes of soluble IL-6 and IL-6R elicit responses from gp130expressing cells that lack cell surface IL-6R. Transsignaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block transsignaling from IL6/IL6R but not from other cytokines that utilize gp130 as a coreceptor.
Source: E. coli
Species: Human
Form: Lyophilized from a 0.2μm filtered solution in PBS containing 0.1% HSA, pH7.4
Bio-activity: Measured in a cell proliferation assay using B9 -11, the specific activity shall be not less than 1 x 10^7 IU/mg.
Endotoxin: ≤1EU/μg, determined by the LAL method.
Purity: ≥97%, as determined by SDS-PAGE and HPLC method.
Storage: Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2- 8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 100μg/ml.
Gene Name: IL6 interleukin 6 [ Homo sapiens ]
Official Symbol: IL6
Synonyms: HGF; HSF; BSF2; IL-6; IFNB2; interleukin-6; B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; IFN-beta-2; hybridoma growth factor; interferon beta-2; interferon, beta 2; interleukin BSF-2
Gene ID: 3569
mRNA Refseq: NM_000600
Protein Refseq: NP_000591
MIM: 147620
UniProt ID: P05231
Chromosome Location: 7p21
Pathway: ARMS-mediated activation, organism-specific biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, conserved biosystem
Function: cytokine activity; growth factor activity; interleukin-6 receptor binding

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