Active Recombinant Human PVRL4 Protein, His-tagged

• Cat.No.: PVRL4-776H
• Product Overview: Recombinant Human PVRL4 (Accession # NP_112178) Gly27-Val351, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gly27
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 x 10^4 cells/well are added to recombinant human Nectin-4 coated plates (10 µg/mL with 100 µL/well), approximately 45% - 65% will adhere after 1 hour incubation at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human PVRL4, His-tagged has a calculated MW of 35.6 kDa. In SDS-PAGE migrates as 44 kDa, reducing conditions.
• Protein length: Gly27-Val351
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PVRL4 poliovirus receptor-related 4 [ Homo sapiens ]
• Official Symbol: PVRL4
• Synonyms: PVRL4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; LNIR; nectin 4; PRR4; Ig superfamily receptor LNIR; EDSS1; nectin-4; DKFZp686K05193
• Gene ID: 81607
• mRNA Refseq: NM_030916
• Protein Refseq: NP_112178
• MIM: 609607
• UniProt ID: Q96NY8
• Chromosome Location: 1q22-q23.2