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Recombinant Rat Nfasc Protein, His-tagged

Cat.No.: Nfasc-797R
Product Overview: Recombinant Rat Nfasc (Accession # NP_446361) Ile25-Ala1031, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Rat
Tag: His
Predicted N Terminal: Ile25
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been pre-coated with 50 µL/well of the rrNeurofascin solution at 10-15 µg/mL.
Molecular Mass: Recombinant Rat Nfasc, His-tagged has a calculated MW of 114.7 kDa. In SDS-PAGE migrates as 135-140 kDa, reducing conditions.
Protein length: Ile25-Ala1031
Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: Nfasc neurofascin [ Rattus norvegicus ]
Official Symbol: Nfasc
Synonyms: NFASC; neurofascin; NF;
Gene ID: 116690
mRNA Refseq: NM_001160313
Protein Refseq: NP_001153785
MIM:

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