Recombinant Human IDH1 Protein, His-tagged IDH1-1044H

Recombinant Human IDH1 Protein, His-tagged


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Recombinant Human IDH1 Protein, His-tagged

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Cat.No. : IDH1-1044H
Product Overview : Recombinant Human IDH1 (Accession # O75874) Met1-Leu414, fused with a C-terminal 6-His tag, was produced in E. coli.
Source : E. coli
Species : Human
Tag : His
Predicted N Terminal : Ser2
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl, Glycerol, Brij-35 and DTT.
Bio-activity : Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate.The specific activity is >10,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human IDH1, His-tagged has a calculated MW of 47 kDa. In SDS-PAGE migrates as 43-45 kDa, reducing conditions..
Protein length : Met1-Leu414
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : IDH1 isocitrate dehydrogenase 1 (NADP+), soluble [ Homo sapiens ]
Official Symbol : IDH1
Synonyms : IDH1; isocitrate dehydrogenase 1 (NADP+), soluble; isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; oxalosuccinate decarboxylase; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; IDH; IDP; IDCD; IDPC; PICD;
Gene ID : 3417
mRNA Refseq : NM_005896
Protein Refseq : NP_005887
MIM : 147700
UniProt ID : O75874
Chromosome Location : 2q32-qter

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