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Recombinant Mouse Lgmn Protein, His-tagged

Cat.No.: Lgmn-1059M
Product Overview: Recombinant Mouse Lgmn (Accession # O89017) Val18-Tyr435, fused with an N-terminal 7-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Mouse
Tag: His
Predicted N Terminal: His
Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >350 pmol/min/µg, as measured under the described conditions.
Molecular Mass: Recombinant Mouse Lgmn, His-tagged has a calculated MW of 49 kDa. In SDS-PAGE migrates as 61 kDa, reducing conditions.
Protein length: Val18-Tyr435
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: Lgmn legumain [ Mus musculus ]
Official Symbol: Lgmn
Synonyms: LGMN; legumain; preprolegumain; protease, cysteine 1; protease, cysteine, 1; asparaginyl endopeptidase; AEP; Prsc1; AI746452; AU022324;
Gene ID: 19141
mRNA Refseq: NM_011175
Protein Refseq: NP_035305
MIM:

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