Active Recombinant Mouse Lgmn Protein, His-tagged

• Cat.No.: Lgmn-1059M
• Product Overview: Recombinant Mouse Lgmn (Accession # O89017) Val18-Tyr435, fused with an N-terminal 7-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >350 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Lgmn, His-tagged has a calculated MW of 49 kDa. In SDS-PAGE migrates as 61 kDa, reducing conditions.
• Protein length: Val18-Tyr435
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Lgmn legumain [ Mus musculus ]
• Official Symbol: Lgmn
• Synonyms: LGMN; legumain; preprolegumain; protease, cysteine 1; protease, cysteine, 1; asparaginyl endopeptidase; AEP; Prsc1; AI746452; AU022324
• Gene ID: 19141
• mRNA Refseq: NM_011175
• Protein Refseq: NP_035305