||Recombinant Mouse Interferon beta 1 protein was expressed in E.coli and purified by using conventional chromatography techniques, 22.0 kDa (182 aa) with his tag.
||Interferon beta 1, also known as IFN-beta, is produced mainly by fibroblasts and some epithelial cell types. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. It also increases the expression of HLA class I antigens and blocks the expression of HLA class II antigens stimulated by IFN-gamma. Interferon beta 1 can be used for topic treatment of condylomata acuminata. Some studies suggest that Interferon beta 1 tends to prevent disease activity in patients with multiple sclerosis.
||Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 0.1 M NaCl, 30% glycerol
||> 95% by SDS - PAGE.
||0.5 mg/ml (determined by Bradford assay)
||< 1.0 EU per 1 μg of protein (determined by LAL method )
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MINYKQLQLQ ERTNIRKCQE LLEQLNGKIN LTYRADFKIP MEMTEKMQKS YTAFAIQEML QNVFLVFRNN FSSTGWNETI VVRLLDELHQ QTVFLKTVLE EKQEERLTWE MSSTALHLKS YYWRVQRYLK LMKYNSYAWM VVRAEIFRNF LIIRRLTRNF QN
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.