Recombinant Resistance to Inhibitors of Cholinesterase, C.elegans

• Cat.No.: Ric-4-259C
• Product Overview: SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques (207 aa, 23 kDa).

Specification

• Cat. No.: Ric-4-259C
• Description: SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release. The membrane proteins SNAP-25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane.
• Source: Escherichia Coli.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 5 mM DTT, 50 mM NaCl, 1 mM EDTA, 10% glycerol.
• Molecular Weight: 23 kDa (207 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Amino acid Sequence: MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERC EGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQP RITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ric-4 Resistance to Inhibitors of Cholinesterase [ Caenorhabditis elegans ]
• Synonyms: SNAP25; FLJ23079; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP; Super-Protein, bA416N4.2; dJ1068F16.2; OTTHUMP00000030267; OTTHUMP00000030268; Super protein; Synaptosomal-associated 25 kDa protein; resistance to inhibitors of cholinesterase 4 homolog; synaptosomal-associated protein 25; synaptosomal associated protein, 25kDa; Synaptosomal-associated protein, 25kDa; SNAP-25 (synaptosome-associated protein of 25,000 daltons).
• Gene ID: 179427
• mRNA Refseq: NM_001129561
• Protein Refseq: NP_001123033
• UniProt ID: O62414
• Chromosome Location: V
• Pathway: SNARE interactions in vesicular transport.