Recombinant Human Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) UCHL1-181H

Cat. No.:	UCHL1-181H
Product Overview:	Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques (223 aa, 24.8 kDa).
Description:	PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson"s disease susceptibility.
Source:	Escherichia Coli.
Formulation:	Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA.
Molecular Weight:	24.8 kDa (223 aa) (Real molecular weight on SDS-PAGE will be shift up).
Purity:	> 95% by SDS – PAGE.
Concentration:	1 mg/ml (determined by Bradford assay).
Amino acid Sequence:	MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA
Storage:	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

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Gene Name:	UCHL1 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) [ Homo sapiens ]
Synonyms:	UCHL1; PARK5; PGP9.5; Uch-L1; UCH-L1; EC 3.4.19.12, EC 6.-.-.-; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; ubiquitin C-terminal esterase L1; ubiquitin carboxyl-terminal esterase L1; ubiquitin thiolesterase L1; Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxy-terminal hydrolase L1; Ubiquitin carboxy-terminal hydrolase L1 (UCH-L1); EC 3.1.2.15; ubiquitin thiolesterase; ubiquitin carboxy-terminal esterase; isopeptidase; isopeptidase T; ubiquitin C-terminal hydrolase; ubiquitin carboxy-terminal hydrolase; ubiquitin-C-terminal-thiolester hydrolase; ubiquitin-C-terminal-thioester hydrolase.
Gene ID:	7345
mRNA Refseq:	NM_004181
Protein Refseq:	NP_004172
MIM:	191342
UniProt ID:	P09936
Chromosome Location:	4p14
Pathway:	Neurodegenerative Disorders; Parkinson"s disease.
Function:	cysteine-type endopeptidase activity; ligase activity; omega peptidase activity; peptidase activity; protein binding; ubiquitin binding; ubiquitin thiolesterase activity.