||Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques (223 aa, 24.8 kDa).
||PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson"s disease susceptibility.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA.
||24.8 kDa (223 aa) (Real molecular weight on SDS-PAGE will be shift up).
||> 95% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
|Amino acid Sequence:
||MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.