Recombinant Human Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase)

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Recombinant Human Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase)

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Cat. No. : UCHL1-181H
Product Overview : Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques (223 aa, 24.8 kDa).
Description : PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson"s disease susceptibility.
Source : Escherichia Coli.
Formulation : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA.
Molecular Weight : 24.8 kDa (223 aa) (Real molecular weight on SDS-PAGE will be shift up).
Purity : > 95% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Amino acid Sequence : MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDBrendering based on 2etl.Available structures:2etl. :

Gene Name : UCHL1 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) [ Homo sapiens ]
Synonyms : UCHL1; PARK5; PGP9.5; Uch-L1; UCH-L1; EC 3.4.19.12, EC 6.-.-.-; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; ubiquitin C-terminal esterase L1; ubiquitin carboxyl-terminal esterase L1; ubiquitin thiolesterase L1; Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxy-terminal hydrolase L1; Ubiquitin carboxy-terminal hydrolase L1 (UCH-L1); EC 3.1.2.15; ubiquitin thiolesterase; ubiquitin carboxy-terminal esterase; isopeptidase; isopeptidase T; ubiquitin C-terminal hydrolase; ubiquitin carboxy-terminal hydrolase; ubiquitin-C-terminal-thiolester hydrolase; ubiquitin-C-terminal-thioester hydrolase.
Gene ID : 7345
mRNA Refseq : NM_004181
Protein Refseq : NP_004172
MIM : 191342
UniProt ID : P09936
Chromosome Location : 4p14
Pathway : Neurodegenerative Disorders; Parkinson"s disease.
Function : cysteine-type endopeptidase activity; ligase activity; omega peptidase activity; peptidase activity; protein binding; ubiquitin binding; ubiquitin thiolesterase activity.

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